My research utilizes functional genomics approaches to uncover and characterize mechanisms of tumorigenesis and tumor maintenance, with the ultimate goal of identifying potential therapeutic targets for cancer. To this end, I use high-throughput technologies coupled with RNAi and open-readingframe (ORF) expression libraries to perform functional characterization in a systematic and comprehensive manner. Current projects include use of loss-of-function RNAi screening approaches to identify genes essential for cancer cell proliferation and survival, as they provide points of vulnerability of the cancer and may translate directly to drug targets. Moreover, by performing synthetic-lethal screens, we can identify those genes that are essential only in specific contexts, for example in the presence of specific oncogenic mutations or aberrantly activated signaling pathways. This approach may be of particular clinical relevance, as it provides both a specific drug target and a biomarker to identify patients most likely to respond, facilitating the development of personalized, molecularly targeted therapy. Other projects currently ongoing include gain-of-function ORF screens to identify (1) modifiers of chemotherapeutic drug response and (2) regulators of signaling pathways commonly activated during tumorigenesis.
