The Caglayan lab in the Department of Biochemistry and Molecular Biology, University of Florida, seek a Postdoctoral Associate with training in biochemistry and/or enzymology to study the DNA repair. The NIH-funded research project will focus on studying genome stability, DNA repair, and DNA damage processing. For more information, please see the lab website at https://caglayan.biochem.med.ufl.edu.
The Caglayan group is in both the Department of Biochemistry and Molecular Biology, and UF Health Cancer Center with investigators renowned for their contributions to the area of biochemistry and cancer biology. The lab has an in-house equipment: GE Typhoon RGB, Amersham AI680, GE AKTA fast protein liquid chromatography (FPLC), RQF-3 KinTek Rapid Quench Flow Instrument. The lab utilizes a multi-disciplinary approach to investigate complex biological questions using X-ray structure (structure-function studies), biochemistry, enzymology, and molecular biology.
Duties will include:
Supporting projects through original research, collaboration with other team members, interpreting and critically evaluating primary literature; collection and analysis of data; and maintaining a complete, accurate, and up-to-date lab notebook; preparing and editing manuscripts for publication as well as applications for grants; attending an annual meeting to disseminate research findings and progress; supervising and training of graduate students (PhD and Master’s level) and undergraduates. Applicants should have excellent communication and problem-solving skills and the ability to work flexible hours depending on specific experiments.
1.Caglayan M. Interplay between DNA polymerases and DNA ligases: Influence on substrate channeling and the fidelity of DNA ligation. Journal of Molecular Biology (2019) 31: 2068 – 2081.
2.*Caglayan M. and Wilson S.H. Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals a promutagenic DNA intermediate during double strand break repair. Nature Communications (2018) 9: 4213. *Corresponding author
3.Caglayan M., Prasad R., Krasich R., Longley M.J., Kadoda K., Tsuda M., Sasanuma H., Takeda S., Tano K., Copeland W.C., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts. Nucleic Acids Research (2017) 17: 10079 – 10088.
4.Caglayan M., Horton J.K., Da-Peng D., Stefanick D.F., Wilson S.H. Oxidized nucleotide insertion by pol β confounds ligation during base excision repair. Nature Communications (2017) 8: 14045.
5.Caglayan M., Wilson S.H. Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair. DNA Repair (2015) 35: 85 – 89.
6.Caglayan M., Horton J.K., Prasad R., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Research (2015) 43: 2271 – 2281.
7.Caglayan M., Batra V.K., Sassa A., Prasad R., Wilson S.H. Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair. Nature Structural and Molecular Biology (2014) 21: 497 – 499.
PhD in a related discipline.
The candidate should have experience in biochemistry (protein purification) and enzymology (rapid quench, enzyme kinetics). Previous working experience with evidence of experimental skills with DNA repair proteins is preferred.